The temple has ancient roots but was rebuilt multiple times, notably by Koch King Naranarayana in the 16th century, resulting in the distinctive Nilachal style of architecture. The dome resembles a beehive, crowned by a series of stupas, giving it a unique appearance. The central shrine houses a natural stone formation that resembles the yoni or female genitalia, symbolizing Goddess Kamakhya’s creative and fertile energy. The stone is veiled by a natural spring.

The temple is open daily from 5:30 am to 1:00 pm. And again from 2:30 pm to 5:30 pm.
The Morning Pooja includes – morning Aarti from around 6:30 am to 7:00 am. Followed by Ashtottara Archana which is chanting of 108 names of the Goddess between 7:00 am to 9:30 am. Rudrabhishek & Navagraha Puja are dedicated to Lord Shiva and the celestial bodies performed between 9:30 am and 12:00 pm.
The temple closes for around an hour between 1:00 pm to 2:00 pm for offering Bhoga (cooked offerings) to the deity.
The Afternoon & Evening Pooja includes evening Aarti around sunset, by 4:30 pm to 6:00pm, involving lamps and hymns along with another round of Ashtottara Archana.
The Ambubachi Mela is a unique festival held at the Kamakhya temple in Assam, drawing thousands of devotees, sadhus, and tourists, and is also a festival that celebrates the annual menstrual cycle of the goddess Kamakhya. During this time, the temple remains closed for three days, making a time of intense spiritual energy and respecting the cycle of creation. And once the three-day period is done, devotees from all over the world, gather to witness the divine phenomenon and seek blessings. The festival emphasis on respect for the natural processes that support life and the divine feminine.